FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCG1323.838325094504216No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1283.722012212852573No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCT1123.256760686246002No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC702.035475428903751No Hit
ATTGCGCAATGAGCATTGTGGGAAGCCAGTGCCCCGCAGCAGTCCAGTGG401.1631288165164293No Hit
ATTGCGCAATGTACGGGGTGGGGGTTAGTTAATGGATACTAAGTTTCTTT300.8723466123873218No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG240.6978772899098575No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAT220.639720849084036No Hit
ATTGCGCAATGTAGAAGTTGGGGCACATGATGCGGCGCACAGCCTGCCAA220.639720849084036No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATG200.5815644082582146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT150.4361733061936609No Hit
ATTGCGCAATGACGCAGGGGGGAGGGCACATTTGCTACGGGTGGTGGTGA150.4361733061936609No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC140.40709508578075027No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.40709508578075027No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACT130.37801686536783946No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA120.34893864495492877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT100.2907822041291073No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG90.2617039837161966No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC90.2617039837161966No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG80.23262576330328583No Hit
ATTGCGCAATGCTCGAGGGGGGACAGTACCGGGAGAATAAGCTCCGGCTA80.23262576330328583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG80.23262576330328583No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC80.23262576330328583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT80.23262576330328583No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATGGATCG80.23262576330328583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC80.23262576330328583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT70.20354754289037513No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC70.20354754289037513No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG70.20354754289037513No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT60.17446932247746438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC60.17446932247746438No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA60.17446932247746438No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC60.17446932247746438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT60.17446932247746438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT60.17446932247746438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT50.14539110206455366No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC50.14539110206455366No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC50.14539110206455366No Hit
ATTGCGCAATGCGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAG50.14539110206455366No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTGATGGATCG50.14539110206455366No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTGT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCTGTC40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGTGCCT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGCGGT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGA40.11631288165164291No Hit
ATTGCGCAATGTGCGACTGGGAAGGGCGTATGGTGGATGCCTAGGGTCTG40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTTAT40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCGG40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC40.11631288165164291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGTGCC40.11631288165164291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)