FastQCFastQC Report
Tue 7 May 2024
EGAF00007822731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10568
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG5335.043527630582892No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT3853.643073429220288No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2252.1290688872066617No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1491.4099167297501891No Hit
ATTGCGCAATGGCAAGAAGGGGAGGAATACATTAACGATTTACTCGTATA970.9178652535957608No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAT750.7096896290688872No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG660.6245268735806208No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTAC600.5677517032551098No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC550.520439061317184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.463663890991673No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG460.43527630582891746No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC430.40688872066616205No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT350.3311884935654807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT330.3122634367903104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT300.2838758516275549No Hit
ATTGCGCAATGATGCATGTGGGATAACCGTCAATACCAAAACACACGATA300.2838758516275549No Hit
ATTGCGCAATGGATCCGAGGAGGCGGAACAAGTCCACGGAGTCCCTGCAG270.2554882664647994No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCGCG260.24602573807721426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.24602573807721426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.24602573807721426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT240.22710068130204392No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCA230.21763815291445873No Hit
ATTGCGCAATGGATGACCTGGGACAAAATAAAACATCAAAAAAACAAATA230.21763815291445873No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACG220.2081756245268736No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.2081756245268736No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT200.18925056775170326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC200.18925056775170326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.18925056775170326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.1797880393641181No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCG180.17032551097653292No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA170.16086298258894777No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.16086298258894777No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATGG160.1514004542013626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT150.14193792581377745No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG150.14193792581377745No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC140.13247539742619227No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTA140.13247539742619227No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC130.12301286903860713No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT130.12301286903860713No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACACTCG130.12301286903860713No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.12301286903860713No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCTCG130.12301286903860713No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.12301286903860713No Hit
ATTGCGCAATGGTATGAACGGGGGGATCAAGCGACTAAGTGCATATGGTG120.11355034065102196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG120.11355034065102196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT120.11355034065102196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.11355034065102196No Hit
ATTGCGCAATGACAGTTCGGGGGGGGATGAGGTGTGATTAGGAGTGAAAA120.11355034065102196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG110.1040878122634368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTTTGCTGCTG110.1040878122634368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG110.1040878122634368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT110.1040878122634368No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG110.1040878122634368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT110.1040878122634368No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)