FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10568
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA10399.831566994700983No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2962.8009084027252085No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1881.7789553368660105No Hit
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGG1401.3247539742619228No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGCC1030.9746404239212717Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTC910.8610900832702498No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTG720.6813020439061317No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCC660.6245268735806208No Hit
ATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGG600.5677517032551098No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGA470.4447388342165026No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.4163512490537472No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC420.39742619227857684No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC380.3595760787282362No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG330.3122634367903104No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG330.3122634367903104No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.2838758516275549No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA280.26495079485238454No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGGCGCC270.2554882664647994Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC250.23656320968962907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.23656320968962907No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.23656320968962907No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGA210.19871309613928842No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC200.18925056775170326No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.1797880393641181No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.1797880393641181No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT190.1797880393641181No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.17032551097653292No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC180.17032551097653292No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTGGCC160.1514004542013626Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA160.1514004542013626No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.1514004542013626No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGGGGTCGCC150.14193792581377745Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG140.13247539742619227No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACA140.13247539742619227No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGGC130.12301286903860713Illumina RNA PCR Primer (95% over 22bp)
ATCTATCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA130.12301286903860713No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATC130.12301286903860713No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA130.12301286903860713No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTC130.12301286903860713No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA130.12301286903860713No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.11355034065102196No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA110.1040878122634368No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT110.1040878122634368No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA110.1040878122634368No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)