FastQCFastQC Report
Tue 7 May 2024
EGAF00007822735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13390
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG5874.38386855862584No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCT4363.2561613144137413No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4223.15160567587752No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2912.173263629574309No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1491.11277072442121No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG760.5675877520537714No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTAT750.5601194921583271No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAAC750.5601194921583271No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC660.4929051530993278No Hit
ATTGCGCAATGACGTGATTGGGGGAAAAGGAAAATAAAGTATAACAAAAG630.4705003734129948No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG620.4630321135175504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT540.4032860343539955No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG410.3061986557132188No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.2987303958177745No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.2987303958177745No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGA350.2613890963405527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT340.25392083644510827No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG330.2464525765496639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC300.22404779686333084No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG280.20911127707244215No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGA280.20911127707244215No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC270.20164301717699776No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCGCG270.20164301717699776No Hit
ATTGCGCAATGGCTTTCGGGGGAAGAGCGTCAAATTCGACTACAAAGTCA250.18670649738610903No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.17923823749066467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC230.1717699775952203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG230.1717699775952203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.15683345780433158No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC210.15683345780433158No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCTCG210.15683345780433158No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.14936519790888725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.14936519790888725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA200.14936519790888725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.14936519790888725No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAA200.14936519790888725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT180.13442867811799852No Hit
ATTGCGCAATGTGTTCGGGGGGGCTCGCGGAAGCTGCGACACAAAAAGAG170.12696041822255413No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.1194921583271098No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.11202389843166542No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT140.10455563853622107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG140.10455563853622107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC140.10455563853622107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)