FastQCFastQC Report
Tue 7 May 2024
EGAF00007822749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17576
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG9545.427856167501138No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT6653.7835685025034134No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3642.0710059171597637No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2481.411015020482476No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT1190.6770596267637687No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAT1160.6599908966772873No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1000.5689576695493855No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTA980.5575785161583978No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC710.40395994538006375No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.392580791989076No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT660.37551206190259445No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG580.32999544833864364No Hit
ATTGCGCAATGTAACTATAGGGGGGGACTCATGGAGAACAGAAATCTCCA580.32999544833864364No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT510.2901684114701866No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.26172052799271733No Hit
ATTGCGCAATGTATCGTAGGGGCGAGCCTGGGGGAACAGGTAGGCATCGA450.2560309512972235No Hit
ATTGCGCAATGCGTCTTGAGGGATGAATGGATGAACGAGATTCCCACTGT450.2560309512972235No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC410.23327264451524807No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA390.22189349112426035No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA390.22189349112426035No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.20482476103777877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC350.19913518434228494No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG350.19913518434228494No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAGCG330.18775603095129723No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT320.18206645425580337No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT300.17068730086481568No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG290.16499772416932182No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.16499772416932182No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT280.15930814747382793No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCGCG270.15361857077833407No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT260.14792899408284024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG260.14792899408284024No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACT250.14223941738734638No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATGG240.13654984069185253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC230.13086026399635867No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT230.13086026399635867No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACGATATCG230.13086026399635867No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT230.13086026399635867No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATC230.13086026399635867No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT220.12517068730086484No Hit
ATTGCGCAATGTCTTGTGAGGGCTTTAGAAATAATTTTGCTAACAATATA220.12517068730086484No Hit
ATTGCGCAATGTGAACGTGGGGGGGCCATGACGATGAACTTGATGCCGCG220.12517068730086484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG210.11948111060537098No Hit
ATTGCGCAATGTCCAGGGGACGAAACCACGTACAACTCGATCATGAAGTG210.11948111060537098No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG200.11379153390987709No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT200.11379153390987709No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.10810195721438325No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.10810195721438325No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.10810195721438325No Hit
TCCGTGCAGGGCATCATCATCTACCGGGCGGCCTACTTCGGCGTGTACGA190.10810195721438325No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT180.10241238051888939No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATCTCG180.10241238051888939No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.10241238051888939No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTCTATCG180.10241238051888939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)