FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17576
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTA5132.9187528447883477No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4932.804961310878471No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGT4922.7992717341829767No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTT4342.469276285844333No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGA3221.8320436959490216No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3161.7979062357760582No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA1180.6713700500682749No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTG1090.6201638598088303No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGG1030.5860263996358671No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.36413290851160673No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG590.33568502503413744No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGG510.2901684114701866No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC500.28447883477469277No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG500.28447883477469277No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG490.2787892580791989No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTG460.26172052799271733No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG440.25034137460172967No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC430.24465179790623576No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA410.23327264451524807No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA400.22758306781975418No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA380.2162039144287665No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC370.21051433773327266No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC360.20482476103777877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC350.19913518434228494No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTC340.19344560764679108No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA330.18775603095129723No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.18206645425580337No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT280.15930814747382793No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA270.15361857077833407No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGGGGT270.15361857077833407No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAG260.14792899408284024No Hit
CCCTCCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA260.14792899408284024No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.11948111060537098No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA200.11379153390987709No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA200.11379153390987709No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAA190.10810195721438325No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA190.10810195721438325No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA190.10810195721438325No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGGGGA180.10241238051888939No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA180.10241238051888939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)