FastQCFastQC Report
Tue 7 May 2024
EGAF00007822755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14155
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG4002.82585658777817No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3712.620981985164253No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT3022.133521723772519No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2341.6531261038502296No Hit
ATTGCGCAATGGGCAAGGGGGGATTCTAGCATCTGGGAACTATACAAAAT1951.377605086541858No Hit
ATTGCGCAATGCTGGACAGGGGTGGCGACATGAAGGTGCTGCGTGGTCAG1591.1232779936418227No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.7417873542917697No Hit
ATTGCGCAATGGGGATCGCGGGGGTTAAGCGACTAAGCGTACACGGTGGA900.6358177322500883No Hit
ATTGCGCAATGTTCGTAGCGGGGATCGCCAAGGCCGATACGCTTACTCTC900.6358177322500883No Hit
ATTGCGCAATGGGCACACGGGGCTCTCTTTCCGGTGGAGGAGTCTGGAGA880.6216884493111975No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAT830.5863652419639703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG720.5086541858000706No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATA680.48039561992228896No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC640.45213705404450727No Hit
ATTGCGCAATGGGAGGACGGGGAGCCACCATCTGGCCTCAGGAAATGGTC570.4026845637583893No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTG550.3885552808194984No Hit
ATTGCGCAATGGGATGGCCGGGATGGACCATTCGACTGTTTGAAACAAAT540.38149063935005295No Hit
ATTGCGCAATGACACGCGGGGGATGATTGGAGGCTACACGCGCTCACGGA470.33203814906393503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT470.33203814906393503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.282585658777817No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCG380.2684563758389262No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA360.2543270929000353No Hit
ATTGCGCAATGCTTTACGAGGGAGAACGAACGCTGGCGGCATGCCTAACA350.24726245143058992No Hit
ATTGCGCAATGAGGGCTGGGGGGCGTTGCTACTGTGATAGTGTAGTCTCG340.24019780996114448No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT330.23313316849169907No Hit
ATTGCGCAATGCAGCTCGTGGGCCGACAACACTTGCCATTTCGGTCTGAA290.20487460261391735No Hit
ATTGCGCAATGTTGTACCGGGGGGGTTCCGTCTTTCCGGACCTGGCCGAG280.1978099611444719No Hit
ATTGCGCAATGCAAGATCGGGGGAGGGGTGACATGCGTCTTCTTGCCAGC270.19074531967502648No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAG270.19074531967502648No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT260.18368067820558107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC230.16248675379724478No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG220.15542211232779937No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG220.15542211232779937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG200.1412928293889085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC200.1412928293889085No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.1412928293889085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC190.1342281879194631No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAGGTTGGATCG190.1342281879194631No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC190.1342281879194631No Hit
ATTGCGCAATGGAAGAGCAGGGGTATGAGGGAGAATACTGAGCGTGAGGT180.12716354645001765No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAT180.12716354645001765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.12716354645001765No Hit
ATTGCGCAATGGCATCCGTGGGAAGGTAGCTGAGATTGACAATGTGGCTG180.12716354645001765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.12009890498057224No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.12009890498057224No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGAGCG170.12009890498057224No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCT170.12009890498057224No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGGTGGATCT170.12009890498057224No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.11303426351112682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.1059696220416814No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTTGGATCGCG150.1059696220416814No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATAGGTT150.1059696220416814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)