FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG7884.702512382884764No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT6243.7238169123351437No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3782.2557737065107117No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2241.3367547890433849No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATAC1240.7399892582204453No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAT1120.6683773945216924No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1100.6564420839052336No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.6504744285970042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1020.6087008414393985No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG1000.5967655308229397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT960.5728949095900221No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC910.5430566330488751No Hit
ATTGCGCAATGATGGATCCGGGGGCAGAGAAGCAAGGCCAGCACAGAGTC850.5072507011994987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT830.49531539058303997No Hit
ATTGCGCAATGACCTGGACGGGTCTGGTCTCAAATCAGGTAGGATCACCC720.4296711821925166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT600.3580593184937638No Hit
ATTGCGCAATGATCCGGGGGGGGGATCTTTTCCTCAGCTGCCGCCAAGGT550.3282210419526168No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT500.29838276541146985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG490.29241511010324045No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA470.28047979948678164No Hit
ATTGCGCAATGAAGGTAGGGGGAAACTTAGCAAAATGATAGCTAATAAAA460.27451214417855224No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCG430.25660917825386403No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC420.25064152294563463No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC410.24467386763740526No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC410.24467386763740526No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC410.24467386763740526No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC390.23273855702094648No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT380.22677090171271708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG360.2148355910962583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC360.2148355910962583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG360.2148355910962583No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATA360.2148355910962583No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCC340.2029002804797995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT330.19693262517157006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT330.19693262517157006No Hit
ATTGCGCAATGTCTCAGGTGGGGGGGAGCCCATGGTTGTGGGTGAGTGAG310.18499731455511131No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG300.1790296592468819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT290.1730620039386525No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG290.1730620039386525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.1730620039386525No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCGCG270.1611266933221937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT250.14919138270573493No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT240.14322372739750552No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC230.13725607208927612No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT220.13128841678104672No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTCTCG220.13128841678104672No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCTCG210.12532076147281732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT200.11935310616458794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG200.11935310616458794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT190.11338545085635854No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT190.11338545085635854No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT190.11338545085635854No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG180.10741779554812915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG180.10741779554812915No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCGC170.10145014023989975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)