FastQCFastQC Report
Tue 7 May 2024
EGAF00007822758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4972.96592468819001No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGT4292.560124127230411No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTA4242.5302858506892645No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTT3442.0528734260309123No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2751.641105209763084No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGA2471.474010861132661No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGG1300.7757951900698216No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTG1040.6206361520558572No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA830.49531539058303997No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG770.4595094587336635No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.3938652503431401No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.3938652503431401No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC590.35209166318553436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA550.3282210419526168No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGG490.29241511010324045No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTG440.26257683356209344No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG430.25660917825386403No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC420.25064152294563463No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC400.23870621232917588No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.23870621232917588No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC400.23870621232917588No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAG370.22080324640448767No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA370.22080324640448767No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTC360.2148355910962583No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA350.20886793578802887No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT350.20886793578802887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG330.19693262517157006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA320.1909649698633407No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT300.1790296592468819No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.1670943486304231No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC270.1611266933221937No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA240.14322372739750552No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA230.13725607208927612No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA200.11935310616458794No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGGGGT200.11935310616458794No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTGT180.10741779554812915No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAA180.10741779554812915No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA170.10145014023989975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)