FastQCFastQC Report
Tue 7 May 2024
EGAF00007822759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642
Total Bases549.9 kbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCG1865.107084019769357No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCT1313.596924766611752No Hit
ATTGCGCAATGTCACGAATGGGGCTGACAAATTTGATGAGAATGCCAAGA752.059308072487644No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC732.0043931905546404No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC491.3454146073585942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG300.8237232289950577No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATG250.6864360241625481No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC230.6315211422295441No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC190.5216913783635365No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAT170.4667764964305327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT170.4667764964305327No Hit
ATTGCGCAATGCGGGGCGCGGGCAACAGAGAAAGATTCCGTCTCAAAAAA160.43931905546403077No Hit
ATTGCGCAATGTTTTCAGCGGGCAGACAAAAAGAAGACAACACCCAATAC160.43931905546403077No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.43931905546403077No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.43931905546403077No Hit
ATTGCGCAATGCATTTGCTGGGAAGGGCGCACGGTGGATGCCTTGGCACT150.41186161449752884No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCG120.3294892915980231No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT110.3020318506315211No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT110.3020318506315211No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG90.2471169686985173No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT90.2471169686985173No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT90.2471169686985173No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC80.21965952773201539No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG80.21965952773201539No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATGGATCG80.21965952773201539No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTAT80.21965952773201539No Hit
ATTGCGCAATGTACACTTCGGGATGTCGACCTCGAAGACTGGCAAGCACG80.21965952773201539No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC70.19220208676551345No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAGCG70.19220208676551345No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCGCG70.19220208676551345No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG70.19220208676551345No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC60.16474464579901155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG60.16474464579901155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT60.16474464579901155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT50.1372872048325096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT50.1372872048325096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGG50.1372872048325096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA50.1372872048325096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGT50.1372872048325096No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATGGATCT50.1372872048325096No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCAGGAGACCCACTTATGGATCG50.1372872048325096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCGGT50.1372872048325096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGGT40.10982976386600769No Hit
ATTGCGCAATGGCTTGCTTGGGCGCGACCTCAGATCAGACGTGGCGACCC40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGG40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG40.10982976386600769No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCTCG40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGTC40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGCT40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCG40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGG40.10982976386600769No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGATCGC40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT40.10982976386600769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT40.10982976386600769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)