FastQCFastQC Report
Tue 7 May 2024
EGAF00007822761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10024
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG4944.928172386272945No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCT3573.56145251396648No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1861.8555466879489224No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1251.2470071827613727No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.8379888268156425No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAT770.7681564245810055No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG740.7382282521947326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG740.7382282521947326No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGT720.7182761372705506No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC510.50877893056664No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT480.4788507581803671No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.3591380686352753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT350.34916201117318435No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG330.32920989624900243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA330.32920989624900243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT320.3192338387869114No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.30925778132482046No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC300.29928172386272944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG280.27932960893854747No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCG230.2294493216280926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.2294493216280926No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC210.20949720670391062No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC210.20949720670391062No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG200.19952114924181963No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.18954509177972864No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.18954509177972864No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCGCG180.17956903431763765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT180.17956903431763765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT170.1695929768555467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT170.1695929768555467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.1695929768555467No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAGCG160.1596169193934557No Hit
ATTGCGCAATGCTCATGTCGGGCAGCCCCGCCGCAGCGGACCCCGAACGC160.1596169193934557No Hit
ATTGCGCAATGGCTTATCTGGGCGAACTTCCATCAGCTTCCAATTAACCA150.14964086193136472No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.14964086193136472No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC140.13966480446927373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT140.13966480446927373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT140.13966480446927373No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCTCG140.13966480446927373No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.12968874700718278No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.12968874700718278No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT120.11971268954509177No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAGCT120.11971268954509177No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG120.11971268954509177No Hit
ATTGCGCAATGATGGTCCGGGGGAGCGAAGCGGGAAGAGCTCAAATTTGA120.11971268954509177No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG110.1097366320830008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)