FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12173
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG5844.797502669843095No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT4183.433828965743859No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3562.9245050521646268No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2331.914072126838084No Hit
ATTGCGCAATGATATTGTAGGGGAAGGTACGCTAGTTACGGTCGGACATC790.6489772447219256No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG710.5832580300665408No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAT690.5668282264026945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.5668282264026945No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA590.48467920808346343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC580.47646430625154035No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGAC570.46824940441961715No Hit
ATTGCGCAATGGGTACACGGGGTCCACGGCATCGAGGGTGCTTACTCCGC530.43538979709192477No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT520.4271748952600017No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.3696705824365399No Hit
ATTGCGCAATGGCTAAGCGGGGGGGGGATGATTATAATGAAATAGGAAGG400.32859607327692436No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.29573646594923186No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT350.28752156411730884No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG350.28752156411730884No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA330.27109176045346256No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.2628768586215395No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCG290.23823215312577017No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG280.23001725129384704No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC270.22180234946192393No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.21358744763000084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.21358744763000084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.21358744763000084No Hit
ATTGCGCAATGAATTGCTCGGGGGATAACTGTGGTAATTCTAGAGCTAAT230.18894274213423148No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT220.1807278403023084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.1807278403023084No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCTCG210.17251293847038526No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG210.17251293847038526No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT200.16429803663846218No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCGCG200.16429803663846218No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.14786823297461593No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT170.13965333114269285No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC170.13965333114269285No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.13965333114269285No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.13143842931076974No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.13143842931076974No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCT160.13143842931076974No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT150.12322352747884663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT140.11500862564692352No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT140.11500862564692352No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATGG130.10679372381500042No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)