FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT48815.501905972045742No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCC2768.767471410419315Illumina Single End PCR Primer 1 (95% over 23bp)
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1745.527318932655654No Hit
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGG742.3506988564167726No Hit
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGG732.318932655654384No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC461.4612452350698857No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC351.1118170266836087No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGGCGCC341.0800508259212198No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA180.5717916137229987No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGGC170.5400254129606099Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGGGGTCGCC110.34942820838627703No Hit
GTGTATAAGAGACAGATTGCGCAATCTGGCTCTTATACACATCTGACGCT80.25412960609911056No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.25412960609911056No Hit
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGTTCTCGG70.22236340533672172No Hit
CATCTGACGCTGCCGACGATACTTCCGGGGTGTAGATCTCGGTGGTCGCC70.22236340533672172Illumina Single End PCR Primer 1 (95% over 23bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1905972045743329No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCG60.1905972045743329Illumina Single End PCR Primer 1 (95% over 22bp)
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGTTCTCGGTGGTCGCC60.1905972045743329No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT60.1905972045743329No Hit
TCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCCGT60.1905972045743329Illumina Single End PCR Primer 1 (96% over 25bp)
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGGGG60.1905972045743329No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG50.1588310038119441No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTGGCC50.1588310038119441No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.1588310038119441No Hit
CACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCG50.1588310038119441Illumina Single End PCR Primer 1 (95% over 21bp)
TATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTG50.1588310038119441No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA40.12706480304955528No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG40.12706480304955528No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC40.12706480304955528No Hit
GTGTATAAGAGACCGATTGCGCAATCTGTCTCTTATACACATCTGACGCT40.12706480304955528No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG40.12706480304955528No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGTTCTC40.12706480304955528No Hit
GTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC40.12706480304955528No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)