FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13651
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA13049.55241374258296No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3692.7030986740898104No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2591.8972969013259102No Hit
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGG2031.48707054428247No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCC1851.3552120723756502Illumina Single End PCR Primer 1 (95% over 23bp)
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC1561.1427734231924402No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTG1010.73987253681049No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGG950.6959197128415501No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT810.5933631235806901No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.52743388762728No Hit
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGG630.46150465167387006No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.38824994505897004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC520.38092447439748006No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC470.34429712109003No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG460.33697165042854005No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGA430.31499523844407No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC420.30766976778258004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG410.30034429712109No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA410.30034429712109No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG390.28569335579811006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG370.27104241447513No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC360.26371694381364No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATC300.21976411984470004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.21243864918321No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT280.20511317852172No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC280.20511317852172No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACT260.19046223719874003No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA220.16116035455278002No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGGCGCC210.15383488389129002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA200.14650941322980002No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA200.14650941322980002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC190.13918394256831002No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA180.13185847190682No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA180.13185847190682No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTG180.13185847190682No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.12453300124533001No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG170.12453300124533001No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA160.11720753058384001No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.10988205992235002No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGGGGTCGCC150.10988205992235002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)