FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16929
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG9225.446275621714218No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT6153.6328194222931063No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2961.7484789414613975No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2251.3290802764486975No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAT1190.7029357906550889No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1090.643865556146258No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTA1060.6261444857936086No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT880.5198180636777128No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC750.4430267588162325No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.4016775946600508No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG550.3248862897985705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT490.28944414909327193No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT440.2599090318388564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA410.2421879614862071No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.236280938035324No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC400.236280938035324No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA370.21855986768267469No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT370.21855986768267469No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCGCG300.177210703526493No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACT300.177210703526493No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG280.1653966566247268No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAGCG270.1594896331738437No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.1535826097229606No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.1476755862720775No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.1476755862720775No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT240.1417685628211944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG240.1417685628211944No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATC240.1417685628211944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC230.13586153937031129No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT230.13586153937031129No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.13586153937031129No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT220.1299545159194282No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT220.1299545159194282No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG220.1299545159194282No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT210.12404749246854509No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCTCG200.118140469017662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT200.118140469017662No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATGG200.118140469017662No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTCTATCG200.118140469017662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG190.11223344556677892No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT190.11223344556677892No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTAGATCG180.10632642211589581No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.10632642211589581No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.10632642211589581No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT170.1004193986650127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)