FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007822785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22367
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG9684.327804354629588No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6362.8434747619260516No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT6292.812178656055796No Hit
ATTGCGCAATGAACGATATGGGGCGTCTCGCCTGCTGAGGCTCCCTGTCT4371.9537711807573657No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC4121.8419993740778826No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.9701792819779139No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1280.5722716501989539No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCA1240.5543881611302365No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAT1140.5096794384584433No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA1030.46049984351947065No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG960.4292037376492154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC830.3710823981758841No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT760.33978629230562885No Hit
ATTGCGCAATGAGATATCTGGGATGCCTCTTCCAAGCGACGACGCCATTC730.32637367550409085No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.31296105870255286No Hit
ATTGCGCAATGAGATCCGCGGGGGAATTGACGGGGGCCTGCACAAGCGGT690.3084901864353735No Hit
ATTGCGCAATGCTTGATTCGGGGGGCGGGTCTTCGGACACCTTCACACTT680.3040193141681942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT640.28613582509947694No Hit
ATTGCGCAATGTGCTAAGCGGGATGGTGACTGGAGGTGCTAACCTAGGAA600.2682523360307596No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC510.22801448562614568No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT490.219072741091787No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA470.21013099655742837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT470.21013099655742837No Hit
ATTGCGCAATGGGGAGGAAGGGGGAAAAAAGAACAGAAGAGAAGAAAGAG470.21013099655742837No Hit
ATTGCGCAATGATCGGCTGGGGGCTGGCTAAGTTCCGGGTGCAGGGCGGC450.2011892520230697No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG440.19671837975589038No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG430.19224750748871106No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT420.1877766352215317No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC420.1877766352215317No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG390.17436401841999374No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCGCG370.16542227388563507No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC360.16095140161845575No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.16095140161845575No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC350.15648052935127643No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAGCG340.1520096570840971No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC300.1341261680153798No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT280.12518442348102116No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA270.12071355121384184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC250.11177180667948315No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGC250.11177180667948315No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.10730093441230384No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT240.10730093441230384No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATGG230.10283006214512451No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG230.10283006214512451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)