FastQCFastQC Report
Tue 7 May 2024
EGAF00007822786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22367
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA19178.570662136182769No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT7933.545401707873206No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC5292.365091429337864No Hit
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGG3691.6497518665891715No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCC3661.6363392497876335Illumina Single End PCR Primer 1 (95% over 23bp)
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC2391.068538471855859No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.6303929896722851No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTG1310.5856842670004918No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGG1300.5812133947333125No Hit
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGG1150.5141503107256226No Hit
GTCTTAATAGCCCGCCACGAGATATGTACGTTCAGGCAGTTACTCATCCA950.42473286538203603No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.4023785040461394No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT880.39343675951178075No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC780.3487280368399875No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG650.29060669736665623No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC610.27272320829793895No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGA590.26378146376358025No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG580.25931059149640096No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG530.2369562301605043No Hit
CCTGTCCTCTGTGGCTGGTGTACGTCTGCCAGTGACTGGTTAGTTCTCAG500.2235436133589663No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA470.21013099655742837No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC420.1877766352215317No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG420.1877766352215317No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACT410.1833057629543524No Hit
GTTCTGAAGCATGCTCACTCTAGCACTGATGGCTCCTGCATTGTCACAAC410.1833057629543524No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC400.17883489068717306No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTC390.17436401841999374No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT390.17436401841999374No Hit
CTGTCTTAATAGCCCGCCACGAGATATGTACGTTCAGGCAGTTACTCATC370.16542227388563507No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.1475387848169178No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGGCGCC320.14306791254973847No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT310.13859704028255912No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTG310.13859704028255912No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATC310.13859704028255912No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.12965529574820048No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT290.12965529574820048No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.12518442348102116No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC280.12518442348102116No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA270.12071355121384184No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT250.11177180667948315No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC240.10730093441230384No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG230.10283006214512451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)