FastQCFastQC Report
Tue 7 May 2024
EGAF00007822803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14250
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6084.266666666666667No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG5523.873684210526316No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCT3612.533333333333333No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3502.456140350877193No Hit
ATTGCGCAATGCCGAGCCGGGGATAATATGAAAGATATATTTCGTGTGTT1020.7157894736842105No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA870.6105263157894737No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.5754385964912281No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAT700.4912280701754386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG680.4771929824561404No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC660.46315789473684216No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACG620.4350877192982456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT540.3789473684210526No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT520.36491228070175435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT430.3017543859649123No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG420.2947368421052632No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC400.2807017543859649No Hit
ATTGCGCAATGCGCAACCAGGGGATCCTTCTGCATGGACAGGCTCTACGG380.26666666666666666No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC330.23157894736842108No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG280.19649122807017544No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAGCG260.18245614035087718No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA260.18245614035087718No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.18245614035087718No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.17543859649122806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.17543859649122806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.16842105263157894No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.1543859649122807No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCGCG220.1543859649122807No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.1543859649122807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT210.1473684210526316No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG210.1473684210526316No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC200.14035087719298245No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.13333333333333333No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT180.12631578947368421No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.11228070175438595No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.11228070175438595No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.11228070175438595No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.10526315789473684No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCTCG150.10526315789473684No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.10526315789473684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)