FastQCFastQC Report
Tue 7 May 2024
EGAF00007822804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14250
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT7805.473684210526316No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4363.0596491228070173No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTA3212.2526315789473683No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTT1841.2912280701754386No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGT1711.2No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGA1350.9473684210526316No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT780.5473684210526315No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC610.4280701754385965No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.42105263157894735No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGG560.3929824561403509No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTG540.3789473684210526No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.3719298245614035No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC450.3157894736842105No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG430.3017543859649123No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA360.25263157894736843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG280.19649122807017544No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT260.18245614035087718No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC260.18245614035087718No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.16842105263157894No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT240.16842105263157894No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.16140350877192983No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.1543859649122807No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG220.1543859649122807No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC210.1473684210526316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC200.14035087719298245No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGT200.14035087719298245No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA190.13333333333333333No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTTGGAGGGTGGT190.13333333333333333No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT190.13333333333333333No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA170.1192982456140351No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA160.11228070175438595No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA160.11228070175438595No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTT160.11228070175438595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)