FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6789
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3384.9786419207541615No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2103.093239063190455No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTA1311.9295919870378553No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTT791.1636470761525999No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGT771.1341876565031668No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGA460.6775666519369569No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT370.5449992635145088No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC360.5302695536897923No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC320.4713507143909265No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.44189129474149363No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTG280.4124318750920607No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG260.3829724554426278No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG240.3535130357931949No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.33878332596847843No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGG220.324053616143762No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.29459419649432905No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.26513477684489617No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG180.26513477684489617No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT150.22094564737074682No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.22094564737074682No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT130.1914862277213139No Hit
GTGAGGGTTTTCGTTAATTAAAGGAATTAACCAAATCTCACGACATTAAC130.1914862277213139No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT130.1914862277213139No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA130.1914862277213139No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC120.17675651789659744No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGT120.17675651789659744No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT110.162026808071881No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG110.162026808071881No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA110.162026808071881No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC100.14729709824716453No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTT100.14729709824716453No Hit
CATCTGACGCTGCCGACGATTGTTGGAGGGTGTAGATCTCGGTGGTCGCC100.14729709824716453Illumina RNA PCR Primer (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA90.13256738842244808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC90.13256738842244808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC90.13256738842244808No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG80.11783767859773163No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAA70.10310796877301517No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGTGGGTGGT70.10310796877301517No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA70.10310796877301517No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA70.10310796877301517No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG70.10310796877301517No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAAT70.10310796877301517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)