FastQCFastQC Report
Tue 7 May 2024
EGAF00007822811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16970
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG6303.7124337065409545No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5223.0760164997053625No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCT3922.3099587507365937No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3402.003535651149087No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACG1040.6128461991750147No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1010.5951679434295816No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA820.48320565704183854No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAT800.4714201532115498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC800.4714201532115498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.45374189746611665No Hit
ATTGCGCAATGACGAGTTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGG750.44195639363582795No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT670.3948143783146729No Hit
ATTGCGCAATGTTCGCACTGGGAGGGAAAAAATCGGCAGACCATTAGTTA650.3830288744843842No Hit
ATTGCGCAATGTAAAGGTTGGGCATGTCTGCCGGCATGGCGCAGTGAGGT590.34767236299351795No Hit
ATTGCGCAATGATCAGGGGGGGACAAAAAAAAGGGAGGCCTCAGACCTGG570.33588685916322925No Hit
ATTGCGCAATGTGCTCTACGGGACGGCCCAGACTCCTACGGGAGGCAGCA560.32999410724808487No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT550.3241013553329405No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC540.3182086034177961No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA480.2828520919269299No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.27106658809664114No Hit
ATTGCGCAATGACATCTAGGGGGAGCTCCGGTGCCTCACCTGCAGCAAAC380.22392457277548616No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC380.22392457277548616No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG370.21803182086034176No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.2121390689451974No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG360.2121390689451974No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAGCG300.1767825574543312No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.1767825574543312No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG290.1708898055391868No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC290.1708898055391868No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC280.16499705362404243No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG280.16499705362404243No Hit
ATTGCGCAATGTCATGGGGGGGAGGGTGGCAAGCACCAGCGTATGCTGGC260.15321154979375368No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT240.14142604596346495No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC230.13553329404832057No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT230.13553329404832057No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC230.13553329404832057No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAC230.13553329404832057No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCT230.13553329404832057No Hit
ATTGCGCAATGTTCATAGGGGGTTGGTGGATATTGGGCAAGGCAGGAAAC200.11785503830288745No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG200.11785503830288745No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA190.11196228638774308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.11196228638774308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG190.11196228638774308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT190.11196228638774308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT180.1060695344725987No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCGCG170.10017678255745434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)