FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007822817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22565
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG9604.254376246399291No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT6963.084422778639486No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4722.091734987812985No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3121.3826722800797695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.6115665854198981No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTA1170.5185021050299136No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1160.514070463106581No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAT1040.46089076002658985No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT1040.46089076002658985No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT720.31907821847994683No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.29692000886328385No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT670.29692000886328385No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA570.2526035896299579No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC570.2526035896299579No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG540.2393086638599601No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT520.23044538001329493No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA510.22601373808996233No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG430.19056060270330158No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC390.1728340350099712No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT380.1684023930866386No Hit
ATTGCGCAATGCGGGTGCTGGGGGCACGGTGAATGCCTTGGCACTAGAAG370.163970751163306No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC370.163970751163306No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT370.163970751163306No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAGCG330.1462441834699756No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATC330.1462441834699756No Hit
ATTGCGCAATGGGGATTAGGGGCAGGGAGAAAAAGAGTAAGAAAAAAGAA330.1462441834699756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT320.14181254154664302No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCGCG310.13738089962331043No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG290.12851761577664525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG290.12851761577664525No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACT290.12851761577664525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT280.12408597385331266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT280.12408597385331266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC280.12408597385331266No Hit
ATTGCGCAATGCTTTGCTGGGGGCACGGTGAATGCCTTGGCACTAGAAGC280.12408597385331266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG280.12408597385331266No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT260.11522269000664746No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCTCG250.11079104808331486No Hit
ATTGCGCAATGGTGACGAGGGGGAAGCACTGCGGATGGGCACGCTCGCTC240.10635940615998227No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATCTCG230.10192776423664969No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.10192776423664969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)