FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007822818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22565
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6372.8229559051628628No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTA5902.614668734766231No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4071.8036782627963661No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTT3761.6662973631730558No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGT3711.6441391535563925No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGA2801.2408597385331266No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGG1100.4874806115665854No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTG880.38998448925326834No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT870.3855528473299358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.3456680700199424No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.34123642809660976No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC760.3368047861732772No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG700.31021493463328165No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA660.2924883669399512No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG620.27476179924662086No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC580.2570352315532905No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG570.2526035896299579No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGT480.21271881231996453No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG430.19056060270330158No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC410.1816973188566364No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA380.1684023930866386No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTG360.15953910923997341No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAG340.15067582539330823No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC330.1462441834699756No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA320.14181254154664302No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT310.13738089962331043No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT280.12408597385331266No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGG270.11965433192998005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA270.11965433192998005No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11522269000664746No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.11079104808331486No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA240.10635940615998227No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTTGGAGGGTGTA240.10635940615998227No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTTGGAGGGTGGT230.10192776423664969No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC230.10192776423664969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)