FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11463
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG4824.204832940765943No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3392.9573410102067523No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT3242.8264852132949487No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2512.1896536683241736No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.7851347814708192No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAT640.5583180668236937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG640.5583180668236937No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAT630.5495943470295734No Hit
ATTGCGCAATGGCGGCGGGGAGTACGATGGGATACAGGACGAGCCCCAGT570.4972520282648521No Hit
ATTGCGCAATGCTTTAAGAGGGGGGCCCATAGCTCAGTTGGTAGAGCAAC570.4972520282648521No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCG410.3576725115589287No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC350.3053301927942074No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC330.287882753205967No Hit
ATTGCGCAATGAGTCAGGGGGGGACATACAGCGTGGTCCCCAGTCCCGCT320.27915903341184684No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA320.27915903341184684No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT310.2704353136177266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT300.26171159382360637No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT260.22681671464712555No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCG260.22681671464712555No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCAGCG220.19192183547064467No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATG210.18319811567652447No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.17447439588240424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.15702695629416383No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTC170.14830323650004362No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.13957951670592342No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT150.13085579691180318No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC150.13085579691180318No Hit
ATTGCGCAATGTCGAAGTCGGGGGTGGCGAACCGAGTGAACTGAAACATC140.12213207711768298No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA140.12213207711768298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC140.12213207711768298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT140.12213207711768298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG140.12213207711768298No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCGCG140.12213207711768298No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.12213207711768298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG140.12213207711768298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG130.11340835732356278No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCTCG130.11340835732356278No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC120.10468463752944254No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGATGTTCGCATCT120.10468463752944254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT120.10468463752944254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)