FastQCFastQC Report
Tue 7 May 2024
EGAF00007822831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13992
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG5704.073756432246999No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT4273.0517438536306463No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2511.7938822184105203No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1651.179245283018868No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.8933676386506575No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG770.550314465408805No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC760.5431675242995998No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTAC700.5002858776443682No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC670.47884505431675245No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAT640.45740423098913663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT570.4073756432246998No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT520.37164093767867357No Hit
ATTGCGCAATGGTCCTCAGGGGTCAAGCGAACAAGTGCATGTGGTGGATG520.37164093767867357No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCG430.30731846769582616No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC420.3001715265866209No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.2858776443682104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT350.2501429388221841No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA340.24299599771297883No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.18582046883933678No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.18582046883933678No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.1786735277301315No Hit
ATTGCGCAATGCTGATAGGGGGGGGTGACGCAGTGAGGTAGCCACGCCAC240.17152658662092624No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACG240.17152658662092624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.17152658662092624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG240.17152658662092624No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG240.17152658662092624No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.17152658662092624No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCGCG230.164379645511721No Hit
ATTGCGCAATGCAATCTAGGGGCCGCTGCGAAGCTGTACCGGAAGGAGCA230.164379645511721No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAAGCG230.164379645511721No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCA220.15723270440251574No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAC220.15723270440251574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.15008576329331044No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT200.1429388221841052No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCT190.13579188107489995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC190.13579188107489995No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATGG190.13579188107489995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.13579188107489995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.12149799885648942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.12149799885648942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC160.11435105774728416No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.11435105774728416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT160.11435105774728416No Hit
ATTGCGTATGCTGCGTAGGGGGCAGTGCCAAGCACACATTATGCTGAAGG150.10720411663807891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.10720411663807891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG150.10720411663807891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT150.10720411663807891No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTA150.10720411663807891No Hit
ATTGCGCAATGGTTCAGCCGGGTGTGTAACAGCTGGCTCTGTGGCGCCTC150.10720411663807891No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATGT150.10720411663807891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT140.10005717552887365No Hit
ATTGCGCAATGTGTGCCCAGGGCTAGCCATGGGCAGGTTGAAGGTTGGGT140.10005717552887365No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCACGGTACAATCTCG140.10005717552887365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)