FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15100
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG6224.119205298013244No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4382.900662251655629No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT3782.5033112582781456No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2961.960264900662252No Hit
ATTGCGCAATGGTGCCGCTGGGGTGTTCTTTATTAATATCCTAACACTCC2131.4105960264900663No Hit
ATTGCGCAATGAAGTGGTCGGGGATCTCCGAGCGAGAGATGCAAGATGGA1961.2980132450331128No Hit
ATTGCGCAATGGTAGGAGCGGGAATTCTTACCCATTAAAGAACAGGGGTG1731.1456953642384107No Hit
ATTGCGCAATGATACTTCGGGGGGGTATCCAGTTTGATGACGCAATTCTC1390.9205298013245032No Hit
ATTGCGCAATGCAACTCATGGGCCCACTGGCTGCTCCGAAAAGCCATCTT1340.8874172185430464No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.847682119205298No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGG1080.7152317880794702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1000.6622516556291391No Hit
ATTGCGCAATGTGGCTCGCGGGGTGACTGAACTCCCCGCGGTCCCTTCCG950.6291390728476821No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT930.6158940397350993No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG770.509933774834437No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAT760.5033112582781457No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT740.490066225165563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT590.39072847682119205No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCG570.3774834437086093No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC560.3708609271523179No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT460.304635761589404No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG440.2913907284768212No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC430.2847682119205298No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC400.26490066225165565No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG380.25165562913907286No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG380.25165562913907286No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCA370.2450331125827815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC350.2317880794701987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA350.2317880794701987No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC320.2119205298013245No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT320.2119205298013245No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.20529801324503313No Hit
ATTGCGCAATGATGTCCAGGGGGATGAGGGTGCGCTCACCAGTGTTGGCT280.18543046357615894No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG270.17880794701986755No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAC250.16556291390728478No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC240.15894039735099338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT240.15894039735099338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT230.152317880794702No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCTCG220.1456953642384106No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCAGCG200.13245033112582782No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA190.12582781456953643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT180.11920529801324503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG180.11920529801324503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.11920529801324503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT180.11920529801324503No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.11258278145695365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.11258278145695365No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC170.11258278145695365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG170.11258278145695365No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACAACCATCGGCATCT170.11258278145695365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.10596026490066225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)