FastQCFastQC Report
Fri 3 Feb 2023
EGAF00007822834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15100
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTA6664.410596026490066No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5653.741721854304636No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3722.463576158940397No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTT1601.0596026490066226No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGA1340.8874172185430464No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGG1000.6622516556291391No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTG780.5165562913907286No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGGT740.490066225165563No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.4768211920529801No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAG540.3576158940397351No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.33774834437086093No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC420.2781456953642384No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.2781456953642384No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC410.271523178807947No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG400.26490066225165565No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.26490066225165565No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG300.1986754966887417No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT300.1986754966887417No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC290.19205298013245034No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.18543046357615894No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT270.17880794701986755No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC260.17218543046357615No Hit
CTTATACACATCTGACGCTGCCGACGATTCAGGTAGGGTGTAGATCTCGG250.16556291390728478No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.15894039735099338No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCA240.15894039735099338No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.152317880794702No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA220.1456953642384106No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.13245033112582782No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGG180.11920529801324503No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.11920529801324503No Hit
AGCTAGGACTGTCGTGTACTAACCGAAAATATTGCGATAAGCGCCCAACC170.11258278145695365No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCAGGTAG160.10596026490066225No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA160.10596026490066225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)