FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7207
Sequences flagged as poor quality0
Sequence length151
%GC61

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG4596.3688081032329675No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT3805.272651588733176No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1512.0951852365755514No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1131.567920077702234No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAT690.9574025253226031No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG680.9435271264048842No Hit
ATTGCGCAATGCGTTAGGGGGGAAGTGCAAGTGGTAGGAGAGCGTTCCAA660.9157763285694464No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAG640.8880255307340086No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG400.5550159567087554No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC380.5272651588733176No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT360.49951436103787983No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT340.4717635632024421No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT330.4578881642847232No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG320.4440127653670043No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT290.40238656861384764No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG260.360760371860691No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.31913417510753433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.30525877618981545No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGC220.30525877618981545No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTA210.29138337727209657No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC190.2636325794366588No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.2636325794366588No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.24975718051893991No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCGCG180.24975718051893991No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.23588178160122106No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCTCG170.23588178160122106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG160.22200638268350215No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC160.22200638268350215No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT160.22200638268350215No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT150.2081309837657833No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.2081309837657833No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.1942555848480644No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGC140.1942555848480644No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.1942555848480644No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAGCG140.1942555848480644No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT110.15262938809490773No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG110.15262938809490773No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT100.13875398917718884No Hit
ATTGCGCAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCT100.13875398917718884No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT100.13875398917718884No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC100.13875398917718884No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCCT90.12487859025946996No Hit
ATTGCGCATGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAG90.12487859025946996No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG90.12487859025946996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG80.11100319134175107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT80.11100319134175107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC80.11100319134175107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT80.11100319134175107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT80.11100319134175107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT80.11100319134175107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)