FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19713
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG9554.844518845431948No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT7363.7335768274742556No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4962.516106122863085No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3501.77547811089129No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATAT1330.6746816821386902No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1300.6594632983310506No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTA1230.6239537361132248No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT1100.5580074062801197No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC990.5022066656521077No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.4920610764470147No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT880.44640592502409576No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT780.39567797899863033No Hit
ATTGCGCAATGTACGCCTCGGGGGGGCAGAGTAGGGCAGAGTAGGGACGA690.35002282757571146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT630.3195860599604322No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC600.3043676761527926No Hit
ATTGCGCAATGTGCATAGTGGGAGACGTTCTCGCCCAGAGTCGCCGCGGT570.28914929234515296No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT560.2840764977426064No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCG450.2282757571145944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA440.22320296251204788No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAT390.19783898949931517No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT390.19783898949931517No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA380.19276619489676863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG380.19276619489676863No Hit
ATTGCGCAATGCGGGCCCGGGGAAATGGAGAAAATCCAAGAGTGAAATGT360.18262060569167554No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC340.17247501648658245No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG340.17247501648658245No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACT330.16740222188403592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC320.16232942728148936No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG320.16232942728148936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG320.16232942728148936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG300.1521838380763963No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT290.14711104347384973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC290.14711104347384973No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCT290.14711104347384973No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCTCG290.14711104347384973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG280.1420382488713032No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT270.13696545426875667No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATC270.13696545426875667No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATCGCG250.12681986506366358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT240.12174707046111702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT240.12174707046111702No Hit
ATTGCGCAATGCAAGCACTGGGGAGTTTGTTGCATTAGACCCGAAACCCG230.11667427585857049No Hit
ATTGCGCAATGAGAGCCAAGGGGAGGAAGAAGCGAATGCGCAGGCTGAAG230.11667427585857049No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.11667427585857049No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTAGATCG210.1065286866534774No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCATCACTATATGG210.1065286866534774No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT200.10145589205093086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC200.10145589205093086No Hit
ATTGCGCAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGA200.10145589205093086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)