FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19713
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTA10565.356871100289149No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6183.1349870643737634No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4402.2320296251204788No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTT3241.64358545122508No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGA1420.7203368335616092No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTG1190.6036625577030387No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTA1070.5427890224724801No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC1020.5174250494597474No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.40075077360117684No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGT750.3804595951909907No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC690.35002282757571146No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.29929488155024603No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG580.29422208694769947No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG530.2688581139349668No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTATAGTGTA470.2384213463196875No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT430.21813016790950135No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG410.20798457870440826No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC410.20798457870440826No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC410.20798457870440826No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA400.20291178410186173No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG390.19783898949931517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA390.19783898949931517No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCGAGTGTA390.19783898949931517No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC360.18262060569167554No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC340.17247501648658245No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCT320.16232942728148936No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA310.15725663267894283No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA300.1521838380763963No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCT300.1521838380763963No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC300.1521838380763963No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTATTGTA290.14711104347384973No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA270.13696545426875667No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG270.13696545426875667No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT240.12174707046111702No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC240.12174707046111702No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA220.11160148125602394No Hit
TTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA210.1065286866534774No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCGAGTGTT210.1065286866534774No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC210.1065286866534774No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA200.10145589205093086No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT200.10145589205093086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)