FastQCFastQC Report
Tue 7 May 2024
EGAF00007822863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13831
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG7145.162316535319211No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCT4413.188489624755983No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3862.790832188561926No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2741.981057045766756No Hit
ATTGCGCAATGCAAGCACTGGGGTACATTGAAAAAGATGCTGCGCTGGAA1220.8820764948304534No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACG1040.7519340611669438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.6940929795387174No Hit
ATTGCGCAATGGCCGGGTGGGGGCCGAAAGCACCCTGCAGTCATTCAGAC820.5928710866893211No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG710.5133395994505097No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAT710.5133395994505097No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA660.4771889234328682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC640.46272865302581156No Hit
ATTGCGCAATGTGTGATAGGGGGGCCAGCTTGCTCTGCACTGAGAGACTC620.448268382618755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT500.3615067601764153No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT490.35427662497288703No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCG490.35427662497288703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT480.3470464897693587No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT450.32535608415877376No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC410.29643554334466055No Hit
ATTGCGCAATGTGCACTAAGGGGCAGACTGACCTAAGACCTATGTAAAAG400.2892054081411322No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC390.28197527293760394No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG360.260284867327019No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAGCG290.20967392090232087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.19521365049526426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG270.19521365049526426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.18798351529173596No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG250.18075338008820765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.17352324488467935No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG230.16629310968115102No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCGCG230.16629310968115102No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.16629310968115102No Hit
ATTGCGCAATGAGGAGCGTGGGAGGAGCCGATGAAGGACGTGGAAATCTG210.15183283927409444No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGAGCT190.13737256886703783No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT190.13737256886703783No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC170.12291229845998121No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.11568216325645289No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.11568216325645289No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.11568216325645289No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.11568216325645289No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC150.10845202805292459No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.10122189284939628No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.10122189284939628No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT140.10122189284939628No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC140.10122189284939628No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT140.10122189284939628No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT140.10122189284939628No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCACGGATCT140.10122189284939628No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT140.10122189284939628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)