FastQCFastQC Report
Tue 7 May 2024
EGAF00007822864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13831
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5103.687368953799436No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTA3942.8486732701901527No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3402.458245969199624No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGT3102.2413419130937746No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTT2792.017207721784397No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGA1991.4387969055021328No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATA1010.7302436555563588No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTG670.4844190586363965No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.4844190586363965No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC610.4410382474152267No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.3687368953799436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC470.33981635456583037No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC470.33981635456583037No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG420.3036656785481889No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG390.28197527293760394No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC350.25305473212349067No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA350.25305473212349067No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG320.23136432651290578No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACG300.21690405610584917No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG280.20244378569879257No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTG280.20244378569879257No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC240.17352324488467935No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGG230.16629310968115102No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC230.16629310968115102No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.1446027040705661No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC200.1446027040705661No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG200.1446027040705661No Hit
GTCTAGAGTATTCTACATGGGAACAAACCTCCTTATCTTTCTGAAGACTG190.13737256886703783No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA180.1301424336635095No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.1301424336635095No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1301424336635095No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.11568216325645289No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT150.10845202805292459No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC150.10845202805292459No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA150.10845202805292459No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAA150.10845202805292459No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.10845202805292459No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA140.10122189284939628No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10122189284939628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)