FastQCFastQC Report
Tue 7 May 2024
EGAF00007822877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11946
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG4613.859032312070986No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3913.2730621128411186No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCT3332.7875439477649424No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2331.9504436631508453No Hit
ATTGCGCAATGGCTTTGACGGGGGAGAAGGAGGTTCAGGAGGAGCTTTAG2241.8751046375355767No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.8956973045370835No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG660.5524861878453038No Hit
ATTGCGCAATGGATTGAGGGGGCAGTGAACGCGAGTACCATACCGAGCCA610.510631173614599No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCG600.5022601707684581No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTAT550.46040515653775327No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCG480.4018081366147665No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.3850661309224845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.3348401138456387No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAAC400.3348401138456387No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT350.29298509961493385No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC330.2762430939226519No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG300.25113008538422904No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT280.23438807969194708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.21764607399966515No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG240.20090406830738325No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGA210.1757910597689603No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC210.1757910597689603No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCT200.16742005692281936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.16742005692281936No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.16742005692281936No Hit
ATTGCGCAATGAATCCACTGGGGTTTGCGCTGTGGTTCGTGCTCACGATC190.1590490540766784No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT180.15067805123053743No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.14230704838439645No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG170.14230704838439645No Hit
ATTGCGCAATGTGACTTTGGGGCGTGAGATGTTGGGTTAAGTCCCGCAAC160.13393604553825547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.12556504269211452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.12556504269211452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.12556504269211452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG150.12556504269211452No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC150.12556504269211452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC140.11719403984597354No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATGG140.11719403984597354No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCGATAACTATCGCG140.11719403984597354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT140.11719403984597354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT140.11719403984597354No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGGTG130.10882303699983258No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.10882303699983258No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.10045203415369162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)