FastQCFastQC Report
Tue 7 May 2024
EGAF00007822878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11946
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTA7666.412188180143981No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5094.2608404486857525No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2962.4778168424577265No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTT1351.1300853842290306No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGA760.6361962163067136No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTG640.5357441821530219No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.46877615938389416No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.45203415369161226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC470.3934371337686255No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA460.3850661309224845No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAG450.3766951280763436No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC380.3180981081533568No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.2846140967687929No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.24275908253808806No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA280.23438807969194708No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGT260.21764607399966515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG260.21764607399966515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.21764607399966515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC260.21764607399966515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.19253306546124224No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGG230.19253306546124224No Hit
CTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTC210.1757910597689603No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.1590490540766784No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGT190.1590490540766784No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC190.1590490540766784No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGA180.15067805123053743No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA180.15067805123053743No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.15067805123053743No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA170.14230704838439645No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA170.14230704838439645No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.14230704838439645No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT170.14230704838439645No Hit
ACCTAGAGCTTCATCAATAATACCATCTATTAAACCAACTTTTAATAAAC170.14230704838439645No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC160.13393604553825547No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.12556504269211452No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA150.12556504269211452No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA150.12556504269211452No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTAC150.12556504269211452No Hit
GCTCCACCTAGAGCTTCATCAATAATACCATCTATTAAACCAACTTTTAA140.11719403984597354No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10882303699983258No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT130.10882303699983258No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGTTCTCGG120.10045203415369162No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA120.10045203415369162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)