FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2866.801426872770511No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTA2315.4934601664684894No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1593.7812128418549347No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTT410.9750297265160524No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.7847800237812128No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGA260.6183115338882282No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA250.5945303210463734No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAG240.5707491082045184No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTG230.5469678953626634No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.47562425683709864No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG180.4280618311533888No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC180.4280618311533888No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC160.380499405469679No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG140.3329369797859691No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA120.2853745541022592No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC120.2853745541022592No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGT110.2615933412604043No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGG100.23781212841854932No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT100.23781212841854932No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT100.23781212841854932No Hit
CATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGGTGGTCGCC100.23781212841854932Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG90.2140309155766944No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG90.2140309155766944No Hit
GGCCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGG90.2140309155766944No Hit
GGGTTGAGCCCCGGGCTTTCACCTCTGACTTAAAAAACCGCCTACGCGCG80.1902497027348395No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA80.1902497027348395No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC80.1902497027348395No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA80.1902497027348395No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT70.16646848989298454No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC70.16646848989298454No Hit
CTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATGTC70.16646848989298454No Hit
ATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGGTGG70.16646848989298454No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA70.16646848989298454No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16646848989298454No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA70.16646848989298454No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA60.1426872770511296No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.1426872770511296No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGT60.1426872770511296No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGA60.1426872770511296No Hit
CTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTC60.1426872770511296No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA60.1426872770511296No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA60.1426872770511296No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA50.11890606420927466No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAA50.11890606420927466No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAATA50.11890606420927466No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG50.11890606420927466No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.11890606420927466No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA50.11890606420927466No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATCTG50.11890606420927466No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA50.11890606420927466No Hit
TCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA50.11890606420927466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)