FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12100
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG4643.834710743801653No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT3522.909090909090909No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC1931.5950413223140496No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1441.1900826446280992No Hit
ATTGCGCAATGCATTCAAGGGGAAGTGAGAACCTTGAAGACTGAAGTGGA1441.1900826446280992No Hit
ATTGCGCAATGGGTCTAATGGGCAGAGTTCGCCAAGCGCTGCTGGTAGCG1311.0826446280991735No Hit
ATTGCGCAATGCTCGCGATGGGCTCACGACAGATGACATTCTTACACAAA1221.0082644628099173No Hit
ATTGCGCATGGTTCAAGGCGCGCTCCAGGCATCTGATGGACTACGATACC1120.9256198347107438No Hit
ATTGCGCAATGCAATACCAGGGGCCGCGCCTGCGACCGAGTCGTCGCATG1120.9256198347107438No Hit
ATTGCGCAATGGGTGCTCAGGGATGGCGGTTACGGAAACCCGTCTAGTGG1030.8512396694214877No Hit
ATTGCGCAATGCAACGATGGGGGTGCTCGGACTCGTGCTTAGCTCCTGCT970.8016528925619835No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAG960.7933884297520661No Hit
ATTGCGCAATGGTGTAGGTGGGACTGAGAAGTTTGCGCGTATGCCACGCA920.7603305785123967No Hit
ATTGCGCAATGGAGGGTGGGGGCGGTTGGATGACGTGTGGACCAATGCGT910.7520661157024793No Hit
ATTGCGCAATGTAGTCCTCGGGCGCGGTTATACGAACTGGGCGAACGTTG800.6611570247933884No Hit
ATTGCGCAATGATATCCAGGGGACTGTATAGTGGATGCGAGTATCTTTAT790.6528925619834711No Hit
ATTGCGCAATGCAAATACCGGGGATGCGACAACAGCTAACTGCCATTCGT770.6363636363636364No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAT740.6115702479338843No Hit
ATTGCGCAATGGAGGCCTAGGGATGGAAAGCGCCAGTCCTGCGCGGGGCA700.5785123966942148No Hit
ATTGCGCAATGGTATCTCAGGGGGGTGGTAGTGGAAATGAGGAGTGTGGA690.5702479338842975No Hit
ATTGCGCAATGTTGGGGCCGGGATATCAATAAGCGGAGGAAAAGAAACTA560.4628099173553719No Hit
ATTGCGCAATGTGGAAGAAGGGGGAGACATACCTCCGTGAGCTCAAGGCG520.4297520661157025No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG510.4214876033057851No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG510.4214876033057851No Hit
ATTGCGCAATGCCTTTACCGGGGCGCCTGGCTGGCAGCAAGGCGGACAAC510.4214876033057851No Hit
ATTGCGCAATGACGGGGCGGGGCGACGCTGACCAAGTATGACACTGTGGT480.39669421487603307No Hit
ATTGCGCAATGACCGGAGCGGGCACGGGCCCGAACGAAGAAGTCGGAAAA470.3884297520661157No Hit
ATTGCGCAATGCAGCCTTGGGGCATGCTGTCGCATTATGAGCTGTGGCAT460.38016528925619836No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCG440.36363636363636365No Hit
ATTGCGCAATGCAACTAGTGGGGCGCAAGGGTACGTATACAGGCACGCGA440.36363636363636365No Hit
ATTGCGCAATGGGTCTGAGGGGCAGATTTTCTCGCAGGCGCTGATGAACT440.36363636363636365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC410.33884297520661155No Hit
ATTGCGCAATGATCGCACGGGGAAGGAGCGTCGCATCGTCATCCTCATTA400.3305785123966942No Hit
ATTGCGCAATGTTCTGGCCGGGGACAAAGATATGAATCTGTACAGCTTTG390.32231404958677684No Hit
ATTGCGCAATGTGCGAGGAGGGGGAGAACAGGATCGTCTCGATTCTGTCG370.30578512396694213No Hit
ATTGCGCAATGCTTCCACTGGGGGTATTATCAAGATGAACGTCGACACCG360.2975206611570248No Hit
ATTGCGCAATGAGGGATGCGGGAAAAAGAAAAGTAAAATCTGTTGTATCT360.2975206611570248No Hit
ATTGCGCAATGACGGATAGGGGGAACGCGGCGAACACGGAGGCCGTGGTC340.28099173553719003No Hit
ATTGCGCAATGTGCGTTACGGGGAGCGAAGCGGGAAGAGCTCAAATTTGA340.28099173553719003No Hit
ATTGCGCAATGAGGGTGGGGGGAAAAGAAAAGTAAAATCTGTTGTATCTA310.25619834710743805No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT300.24793388429752067No Hit
ATTGCGCAATGAACTGAGGGGGATCTACGACTTTGAGTTTCAGAAGGGTG290.2396694214876033No Hit
ATTGCGCAATGCTAGGGTCGGGGAGGAGTTTACGGGCCTGGTGATGGCCC290.2396694214876033No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT280.23140495867768596No Hit
ATTGCGCAATGTATCCAAGGGGCACGGGGAGGTAGTGACAATATGTAACA280.23140495867768596No Hit
ATTGCGCAATGACCTCCGGGGGCGGGTGCAGTGAAGCCGAACCGTACCAG280.23140495867768596No Hit
ATTGCGCAATGGGGGATACGGGGCCAGCAGACGCGGTTATACGAACTGGG270.2231404958677686No Hit
ATTGCGCAATGATGCGCCGGGGCCGGTGGCTCTGGTGGTGCCGCTGCTGG270.2231404958677686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT270.2231404958677686No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCT260.21487603305785125No Hit
ATTGCGCAATGTTGTAGCAGGGCCGACGGATCTCGACGATGTGATCAGGA260.21487603305785125No Hit
ATTGCGCAATGTAATTCCGGGGTAACGACAAACAGTGTGAAAGACACTGT260.21487603305785125No Hit
ATTGCGCAATGACAATCCGGGGGGTTTTGGAAGTGAGATCTGCGCTCAGG250.2066115702479339No Hit
ATTGCGCAATGCTCTTGTCGGGGGGATGGACGGACGGGCCGATTTATTGC240.19834710743801653No Hit
ATTGCGCAATGAGGTAGGCGGGGGCTCTTGCTCCTAGCAGCATGAAGGTC240.19834710743801653No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG240.19834710743801653No Hit
ATTGCGCAATGGAATAGAGGGGACAAAATCTTAAATCACGAGATCGATCC230.19008264462809918No Hit
ATTGCGCAATGAGTCGCGGGGGATGGTGGCCCATGTTTAATGCCGACGAG230.19008264462809918No Hit
ATTGCGCAATGATTAAGAGGGGAGGGTGATGATGGTCTTTACCACGGCAC220.18181818181818182No Hit
ATTGCGCAATGACGAGCCCGGGAAGGATTCCCCTAGTAACGGCGAGCGAA210.17355371900826447No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT210.17355371900826447No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.17355371900826447No Hit
ATTGCGCAATGGCACGCATGGGATCTGGCCACGACACGGAGGCATTCTGC200.1652892561983471No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.1652892561983471No Hit
ATTGCGCAATGCGATTCCCGGGCTGATAACCCTGGTGCGTGGATGATGCA200.1652892561983471No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTA200.1652892561983471No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGC190.15702479338842976No Hit
ATTGCGCAATGCCCAGTCCGGGAAGGATTCCCCTAGTAACGGCGAGCGAA190.15702479338842976No Hit
ATTGCGCAATGACTTCCCTGGGGAAAGACGTCTGCCAAGACATGAGGCTG190.15702479338842976No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA190.15702479338842976No Hit
ATTGCGCAATGGGAAGAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAA190.15702479338842976No Hit
ATTGCGCAATGGATAGGTCGGGAGGGCAAGCGCTCGCAGCTTCCCTTTGA190.15702479338842976No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.1487603305785124No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC180.1487603305785124No Hit
ATTGCGCAATGGCTCACTGGGGGCGCGTGCCATGCATGAGGCGAAGCTGG180.1487603305785124No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG170.14049586776859502No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14049586776859502No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCGCG160.1322314049586777No Hit
ATTGCGCAATGGAGGGGAGGGGACAAAATCTTAAATCACGAGATCGATCC160.1322314049586777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.1322314049586777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC150.12396694214876033No Hit
ATTGCGCAATGTCCATCCGGGGATGACGGCGCTCTTTCACTTCCAGTCGC150.12396694214876033No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.12396694214876033No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC140.11570247933884298No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAAGCG140.11570247933884298No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCG140.11570247933884298No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG140.11570247933884298No Hit
ATTGCGCAATGGAACAGCGGGGGGCGTCCGCGTTGTAATCTCGAGACGTG130.10743801652892562No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGCGCTTAGAATCTCG130.10743801652892562No Hit
ATTGCGCAATGACCGGAGCGGGCCCGGGCCCGAACGAAGAAGTCGGAAAA130.10743801652892562No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT130.10743801652892562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)