FastQCFastQC Report
Tue 7 May 2024
EGAF00007822890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12100
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTA6905.702479338842975No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2742.2644628099173554No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1791.4793388429752066No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTT1261.0413223140495869No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAG950.7851239669421488No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGA780.6446280991735537No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTG770.6363636363636364No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA550.45454545454545453No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.24793388429752067No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGT290.2396694214876033No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.2231404958677686No Hit
GCCAAGGGCGGAAGGTGTAGCTTGACATAGAAGATCAACAAGAGATGAAA230.19008264462809918No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC220.18181818181818182No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.18181818181818182No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG210.17355371900826447No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGA210.17355371900826447No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.17355371900826447No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGT200.1652892561983471No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT200.1652892561983471No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC190.15702479338842976No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.1487603305785124No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1487603305785124No Hit
GTATATACCATTCGTTTCAATTGTGAAGGTGACCATGAATTGTGTAATTT170.14049586776859502No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGG170.14049586776859502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC160.1322314049586777No Hit
GTGTTACACATACAGCCTTTCGCTTTCCCTCTGATTATTTTCTCATATTA160.1322314049586777No Hit
CCTTCTTCCCCTTCCCAATTTCTCATAACATTATTCCAATCCCTTCAAAC140.11570247933884298No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA130.10743801652892562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG130.10743801652892562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA130.10743801652892562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA130.10743801652892562No Hit
GTCTAATGTGGAGGCTATTTCCTGGAACCCCGTCGAGGCCCGACCAATCC130.10743801652892562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)