FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17954
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTA14067.831123983513423No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4732.634510415506294No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2741.5261223125765846No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTT2321.2921911551743346No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGA1600.891166313913334No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTG1440.8020496825220007No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAG1000.5569789461958339No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA950.5291299988860421No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.43444357803275035No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.32861757825554194No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG570.31747799933162524No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC560.31190820986966694No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG530.29519884148379194No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA520.28962905202183353No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG480.26734989417400024No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT380.21165199955441683No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC370.2060822100924585No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG350.19494263116854182No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGG350.19494263116854182No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA340.1893728417065835No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC340.1893728417065835No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA340.1893728417065835No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGT340.1893728417065835No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA320.17823326278266682No Hit
CTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTC320.17823326278266682No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGA310.17266347332070847No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGA310.17266347332070847No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC310.17266347332070847No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGT300.16709368385875015No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC280.15595410493483347No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT250.13924473654895847No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA250.13924473654895847No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA230.12810515762504177No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA230.12810515762504177No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT220.12253536816308344No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA210.1169655787011251No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGTTCTCGG210.1169655787011251No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT210.1169655787011251No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC210.1169655787011251No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA200.11139578923916675No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTA200.11139578923916675No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGTTGTCGG190.10582599977720841No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG190.10582599977720841No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTAC180.10025621031525009No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10025621031525009No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10025621031525009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)