FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17897
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG8054.497960552047829No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT5332.9781527630329108No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3802.1232608817120187No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2571.4359948594736547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.6481533217857741No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAT1100.6146281499692685No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCA1100.6146281499692685No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1090.6090406213331843No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA930.5196401631558362No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC900.5028775772475834No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT690.38553947588981397No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG670.3743644186176454No Hit
ATTGCGCAATGTAGGAGGAGGGGGTGGGGTGACTGCGTACCTTTTGTATA660.36877688998156116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT640.35760183270939266No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT630.3520143040733084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC460.25702631725987596No Hit
ATTGCGCAATGCATCAACGGGGGAAATTGGTCATTTTAAAATTCAGAATG420.2346762027155389No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC410.22908867407945466No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT380.21232608817120185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA380.21232608817120185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC370.2067385595351176No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG320.17880091635469633No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT310.17321338771861206No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAGCG290.16203833044644353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT280.15645080181035928No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCGCG270.150863273174275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.150863273174275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC270.150863273174275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.14527574453819075No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCAGATCG260.14527574453819075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT250.13968821590210648No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT250.13968821590210648No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.13968821590210648No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.13410068726602223No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG240.13410068726602223No Hit
ATTGCGCAATGTATGACTCGGGGAAAGACCCCGTGCACCTTTACTGTAGC240.13410068726602223No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG240.13410068726602223No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.13410068726602223No Hit
ATTGCGCAATGCCTAGGGAGGGGTATCACGACGCAAAATATCATATTTTC230.12851315862993798No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATGG220.1229256299938537No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT210.11733810135776945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT210.11733810135776945No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA210.11733810135776945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT210.11733810135776945No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGC200.1117505727216852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT190.10616304408560093No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC190.10616304408560093No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT180.10057551544951668No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG180.10057551544951668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)