FastQCFastQC Report
Tue 7 May 2024
EGAF00007822909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13762
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG6684.853945647434966No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT4563.3134718790873423No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2411.7511989536404593No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1911.387879668652812No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAT840.6103763987792472No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGAC830.6031100130794943No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG720.5231797703822119No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA690.501380613282953No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT600.4359831419851766No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC570.4141839848859178No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.3923848277866589No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCG530.385118442086906No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCG470.3415201278883883No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.3051881993896236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT370.2688562708908589No Hit
ATTGCGCAATGCAAACGCCGGGGTCTTCTTGATCGTATCATGGCCATGCT370.2688562708908589No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG320.23252434239209416No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCT310.22525795669234125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.22525795669234125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.22525795669234125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC310.22525795669234125No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC270.19619241389332945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG260.1889260281935765No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATCGCG260.1889260281935765No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.18165964249382358No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA240.17439325679407064No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGATGG230.16712687109431767No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG230.16712687109431767No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATGGACGAGCT220.15986048539456474No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT210.1525940996948118No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.14532771399505887No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT180.13079494259555297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT180.13079494259555297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG180.13079494259555297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC180.13079494259555297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT180.13079494259555297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.12352855689580003No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.12352855689580003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT160.11626217119604708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.10899578549629416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG150.10899578549629416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGTC140.10172939979654119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)