FastQCFastQC Report
Tue 7 May 2024
EGAF00007822921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22276
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCG14006.2847908062488775No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCT7563.3937870353743937No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5332.3927096426647516No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3571.6026216555934638No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCG2751.2345124797988867No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTAT2120.9516968935176873No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGC1770.7945771233614652No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA1400.6284790806248878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.4938049919195547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG950.42646794756688816No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCT870.39055485724546596No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT670.30077213144191056No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCAC670.30077213144191056No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCGATCG610.27383731370084397No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG610.27383731370084397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC600.2693481774106662No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTAGCG580.26036990483031064No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC520.23343508708924404No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC490.2199676782187107No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATGG490.2199676782187107No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCGCG470.21098940563835517No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.20201113305799964No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTCTCG440.19752199676782187No Hit
ATTGCGCAATGTGCGGCTAGGGGGATCAAGCGACTAAGTGCATATGGTGG430.1930328604776441No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT400.1795654516071108No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT400.1795654516071108No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC400.1795654516071108No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA390.17507631531693302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG360.16160890644639972No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTAGCT330.14814149757586642No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG320.14365236128568865No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAG320.14365236128568865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC300.1346740887053331No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCA290.13018495241515532No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATCTCG290.13018495241515532No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGGTATCG280.12569581612497754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC280.12569581612497754No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT260.11671754354462202No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCTATGT260.11671754354462202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG250.11222840725444426No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG250.11222840725444426No Hit
ATTGCGCAATGTCATGTAGGGGGGATCAAGCGACTAAGTGCATATGGTGG250.11222840725444426No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCACCAGCGATCT230.1032501346740887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)