FastQCFastQC Report
Tue 7 May 2024
EGAF00007822922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22276
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGT11465.144550188543724No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGG11315.077213144191058No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6813.057101813611061No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC5132.302926916861196No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTT2431.090860118513198No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTG2421.0863709822230203No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTA2411.0818818459328425No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAG2090.9382294846471538No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTG1980.8888489854551984No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGA1880.8439576225534207No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT1380.6195008080445322No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.5386963548213324No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC870.39055485724546596No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG840.3770874483749327No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG760.3411743580535105No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG710.31872867660262166No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC670.30077213144191056No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG620.2783264499910217No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.24241335966959957No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT510.22894595079906627No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA490.2199676782187107No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGC490.2199676782187107No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC470.21098940563835517No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTA450.20201113305799964No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC440.19752199676782187No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGG410.18405458789728857No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA390.17507631531693302No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGA380.17058717902675524No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA360.16160890644639972No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC340.15263063386604417No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT320.14365236128568865No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA310.13916322499551084No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT300.1346740887053331No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGGGGGGGGGG300.1346740887053331No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC300.1346740887053331No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGT280.12569581612497754No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGG260.11671754354462202No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA250.11222840725444426No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA250.11222840725444426No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGG240.10773927096426647No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA240.10773927096426647No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGGGGGGGGGG230.1032501346740887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA230.1032501346740887No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGG230.1032501346740887No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA230.1032501346740887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)