FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7421
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG4215.673089880070072No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT2793.759601131922921No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2423.26101603557472No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1441.9404392938957011No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAT610.821991645330818No Hit
TGTCTGTGCTGGACGTGTTGTGGGAGGACCGCGACGTGCGCTTCGACGTG570.768090553833715No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTT510.6872389165880609No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC480.6468130979652338No Hit
ATTGCGCAATGTATAAGTGGGGGGACGCGAGCATCTTAAGAACAGGTGCC410.5524861878453038No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCG390.5255356420967525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG380.5120603692224768No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.40425818622827114No Hit
ATTGCGCAATGCTGTTAGGGGGACAAGCGACATTCTCGCTTACAACGACG270.363832367605444No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAAC220.2964560032340655No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.2695054574855141No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.2695054574855141No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCGCG200.2695054574855141No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT180.24255491173696264No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCT180.24255491173696264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT180.24255491173696264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC180.24255491173696264No Hit
ATTGCGCAATGAAGTCCCTGGGGACGGAAAGACCCCGTGCACCTTTACTG160.21560436598841126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA150.20212909311413557No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAA150.20212909311413557No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAAGCG150.20212909311413557No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGT120.16170327449130845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT120.16170327449130845No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.14822800161703276No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGGTAATCG110.14822800161703276No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGGTAATCT110.14822800161703276No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTCATCG110.14822800161703276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG110.14822800161703276No Hit
ATTGCGCAAAAAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGA90.12127745586848132No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACAGGTTAATCTCG90.12127745586848132No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT90.12127745586848132No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG90.12127745586848132No Hit
ATTGCGCAATGGATCGGGAGGGGGGTCAAGTGAATAAGTGCATATGGTGG80.10780218299420563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCGGCCT80.10780218299420563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC80.10780218299420563No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC80.10780218299420563No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAACA80.10780218299420563No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA80.10780218299420563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)