FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13278
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4263.2083145051965656No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2942.214188883868052No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG2662.003313752071095No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG1761.3255008284380179No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT1180.8886880554300347No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAT680.512125320078325No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCA660.49706281066425667No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT660.49706281066425667No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG570.42928151830094896No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA540.4066877541798464No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.391625244765778No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG350.2635939147461967No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC330.24853140533212834No Hit
ATCTGTGGCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG290.21840638650399155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT270.2033438770899232No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT270.2033438770899232No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCAGATCG230.1732188582617864No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT210.15815634884771804No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATGG210.15815634884771804No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA210.15815634884771804No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.15815634884771804No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATGG190.14309383943364964No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAGCG190.14309383943364964No Hit
GGGGGCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180.13556258472661548No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.12803133001958125No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAGCG170.12803133001958125No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG160.12050007531254707No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC150.11296882060551287No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA150.11296882060551287No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGC150.11296882060551287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)