FastQCFastQC Report
Tue 7 May 2024
EGAF00007822934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13278
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5263.961439975899985No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGG4253.2007832504895313No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGT4193.1555957222473263No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3812.869408043380027No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTT880.6627504142190089No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTG850.6401566500979063No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAG820.6175628859768036No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT710.5347190841994276No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTA660.49706281066425667No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTG640.4820003012501883No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGA600.4518752824220515No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG550.4142190088868805No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC550.4142190088868805No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC530.3991564994728121No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.3163126976954361No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGGGGGGGGGG360.27112516945323095No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA360.27112516945323095No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.27112516945323095No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC350.2635939147461967No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG350.2635939147461967No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGG350.2635939147461967No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGGGGGGGGGG350.2635939147461967No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.23346889591805997No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.21087513179695738No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT270.2033438770899232No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGG270.2033438770899232No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.1882813676758548No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC240.18075011296882063No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT240.18075011296882063No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.1732188582617864No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGG230.1732188582617864No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA220.16568760355475223No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA220.16568760355475223No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGTGGG200.15062509414068384No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGA200.15062509414068384No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.15062509414068384No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGG190.14309383943364964No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTG180.13556258472661548No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGTGGGGGGGGGGG170.12803133001958125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC170.12803133001958125No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTAGATCTCGG160.12050007531254707No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGGGGGGGGGG150.11296882060551287No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGTAGATCTCGGTGGTCGCC150.11296882060551287Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA150.11296882060551287No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGG150.11296882060551287No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT150.11296882060551287No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGC150.11296882060551287No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC140.10543756589847869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)