FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17628
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG8845.014749262536873No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT6863.8915361924211482No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4172.365554799183118No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2721.5429997730882687No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAT1440.8168822328114363No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC1260.7147719537100068No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1240.7034263671431813No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATAC1180.6693896074427048No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCG1110.6296800544588155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.4538234626730202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC770.43680508282278196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT690.39142273655547993No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCT640.36305877013841614No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT610.3460403902881779No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC580.32902201043793966No Hit
ATTGCGCAATGTTCTGTCGGGGGCCTTTTGGCTCTCCGGTCGGCACCATG560.31767642387111417No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT530.30065804402087587No Hit
ATTGCGCAATGAACTTGCAGGGATAGCTTTTCTATCGGTAGAGGAGCGTT470.26662128432039933No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG430.24393011118674837No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATA430.24393011118674837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA410.23258452461992285No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG400.2269117313365101No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCC390.22123893805309736No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCG390.22123893805309736No Hit
ATTGCGCAATGAGGTGTACGGGCAGCAGCAGCAACAACAGAAAGGAGGCA380.21556614476968458No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT370.20989335148627183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC340.1928749716360336No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.1928749716360336No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT330.18720217835262082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC320.18152938506920807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT310.17585659178579532No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG290.16451100521896983No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.1531654186521443No Hit
ATTGCGCAATGTTGGAACCGGGGGGGCTGACCGGTTTTGACAGCGGGTAG270.1531654186521443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG270.1531654186521443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.14749262536873156No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC240.13614703880190604No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT230.13047424551849332No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTCTCG230.13047424551849332No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC230.13047424551849332No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCGCG220.12480145223508056No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT220.12480145223508056No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTAGCG210.1191286589516678No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT210.1191286589516678No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGC210.1191286589516678No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT200.11345586566825505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC200.11345586566825505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.11345586566825505No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACGATCG190.10778307238484229No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC190.10778307238484229No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180.10211027910142954No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT180.10211027910142954No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.10211027910142954No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCCATACTATCTCG180.10211027910142954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)