FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17628
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGG6843.880190605854323No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGT6413.6362604946675745No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5513.1257090991604266No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3441.951440889493987No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTG1500.8509189925119129No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAG1380.7828454731109599No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTT1350.7658270932607215No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTA1310.7431359201270706No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTG1220.6920807805763558No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGA1200.6807351940095302No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT1200.6807351940095302No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC730.414113909689131No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA660.37440435670524164No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG580.32902201043793966No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.32902201043793966No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG570.3233492171545269No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG470.26662128432039933No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.26094849103698664No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC450.2552756977535739No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGA410.23258452461992285No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC390.22123893805309736No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGC340.1928749716360336No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC320.18152938506920807No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG310.17585659178579532No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT300.17018379850238255No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGG290.16451100521896983No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC290.16451100521896983No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGG280.15883821193555708No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGGGGGGGGGG270.1531654186521443No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.14749262536873156No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA260.14749262536873156No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA260.14749262536873156No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA250.14181983208531881No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC250.14181983208531881No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT240.13614703880190604No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGG240.13614703880190604No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT220.12480145223508056No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTA210.1191286589516678No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGG210.1191286589516678No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGG210.1191286589516678No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA190.10778307238484229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)