FastQCFastQC Report
Tue 7 May 2024
EGAF00007822939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4031
Total Bases608.6 kbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCG2035.0359712230215825No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCT1654.093277102455966No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC781.935003721161002No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC400.9923096005953859No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC320.7938476804763086No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAT310.769039940461424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG260.6450012403870008No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATG230.5705780203423468No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC180.4465393202679236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT160.3969238402381543No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC140.347308360208385No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAGCG140.347308360208385No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATGGATCG130.3225006201935004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3225006201935004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT110.27288514016373105No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA100.24807740014884647No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100.24807740014884647No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTAT100.24807740014884647No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG90.2232696601339618No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA90.2232696601339618No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT90.2232696601339618No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG80.19846192011907715No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCTCG80.19846192011907715No Hit
ATTGCGCAATGTGGTTCGGGGGGCCCTTTTCCCTTTCTGCCACCGCCATG80.19846192011907715No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC80.19846192011907715No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCGCG70.1736541801041925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT70.1736541801041925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT70.1736541801041925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT70.1736541801041925No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGATCG70.1736541801041925No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCAGGAGACCCACTTATGGATCG70.1736541801041925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC70.1736541801041925No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG70.1736541801041925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG60.14884644008930786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT60.14884644008930786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC60.14884644008930786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC60.14884644008930786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT60.14884644008930786No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATGGAGCT60.14884644008930786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT60.14884644008930786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG60.14884644008930786No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACT60.14884644008930786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC50.12403870007442323No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT50.12403870007442323No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTCTGGATCT50.12403870007442323No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCGGCCT50.12403870007442323No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTG50.12403870007442323No Hit
ATTGCGCAATGAGGATGTCGGGGGGTCTCGAGGGCAGCGATAACAACAAT50.12403870007442323No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)