FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007822941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22622
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG8243.642471929979666No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6883.041287242507294No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCT5532.4445230306781007No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC4542.0068959420033594No Hit
ATTGCGCAATGCAGACGATGGGGTCCGAACCCACACCTGTTGAAAAAGGT2941.2996198390946865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.7426399080541066No Hit
ATTGCGCAATGATGGTTCAGGGGTTCCAGGAAATAGCCCCTCCATTATAG1650.7293784811245689No Hit
ATTGCGCAATGGCACTGCGGGGGGGGATGGTATGGCTCAACGGGGAGAGC1570.6940146759791354No Hit
ATTGCGCAATGCGGGAAAGGGGGGCAGAAGAAGGAGGAAGCAGAGAAAAG1570.6940146759791354No Hit
ATTGCGCAATGATGATCACGGGGGGATATAAAGGAATGATAAGGGAGAAA1430.6321280169746265No Hit
ATTGCGCAATGGGAGGGGGGGAGTAGGCGAGAGGCACCTGGATAAGAGGC1420.6277075413314472No Hit
ATTGCGCAATGTGCGTAGCGGGCACGTGCTGTTGGTGTCATTGTGCACAA1370.6056051631155512No Hit
ATTGCGCAATGATGCAATGGGGGATGATATGTACCATATACATCTTGAGA1320.5835027848996552No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1290.5702413579701175No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCA1130.49951374767925033No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1130.49951374767925033No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA940.4155247104588454No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT900.3978428078861286No Hit
ATTGCGCAATGACGTTGATGGGGCTCCAACAACGGCGTTTAATTTAGGCG880.3890018565997701No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAT870.3845813809565909No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT800.35363805145433647No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT720.31827424630890283No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG590.26080806294757314No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC540.23870568473167714No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA500.22102378215896032No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATCG480.21218283087260187No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC470.2077623552294227No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG460.2033418795862435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT440.19450092829988505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC410.18123950137034744No Hit
ATTGCGCAATGATCGAATCGGGGGGGGGGGAATGGAAAGGTTATAATAAA410.18123950137034744No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC410.18123950137034744No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAT410.18123950137034744No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG390.17239855008398902No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG370.16355759879763063No Hit
ATTGCGCAATGCTCTGCCCGGGAAGTGAATAAGTGCACATGGTGGATGCC370.16355759879763063No Hit
ATTGCGCAATGTAACATCCGGGAGGCCCCACATTTGGGCAACCATTTGTG360.15913712315445142No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC330.1458756962249138No Hit
ATTGCGCAATGATCGAATCGGGGGGGGGGGGAATGGAAAGGTTATAATAA300.13261426929537617No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC290.12819379365219696No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATAGCG260.11493236672265934No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCATATGG250.11051189107948016No Hit
ATTGCGCAATGGATCCCATGGGCGTGGCGGCGACGACCCATTCGAACGTC250.11051189107948016No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT250.11051189107948016No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC250.11051189107948016No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACATCGCAGATCG230.10167093979312175No Hit
ATTGCGCAATGAATCGGGGGGGTAGAAGAAGGCGAGAATGGCACAGTGAC230.10167093979312175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)