FastQCFastQC Report
Tue 7 May 2024
EGAF00007822943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14268
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG6604.625735912531539No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCT4573.202971684889263No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3902.7333894028595456No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2061.4437902999719652No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGT1070.7499299130922343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG950.6658256237734792No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAT920.6447995514437903No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG730.5116344266890944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.46958228202971686No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC640.448556209700028No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT620.43453882814690215No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT600.42052144659377627No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCG560.3924866834875245No Hit
ATTGCGCAATGTCACGAGGGGGGCGTGGCAGGCCATGGCATATTCATCTG520.3644519203812728No Hit
ATTGCGCAATGAGCGGACCGGGTCAGCATCAGTAACTGGGAGACGAAAGC460.32239977572189515No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.3153910849453322No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT430.3013737033922063No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA420.2943650126156434No Hit
ATTGCGCAATGAGGTGGGAGGGCAAGATGGTCAGTAGAACAGAGGGTGCC410.28735632183908044No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.2523128679562658No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG330.23128679562657695No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG330.23128679562657695No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGC330.23128679562657695No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCT310.21726941407345107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG310.21726941407345107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC300.21026072329688814No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTC290.20325203252032523No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCG270.18923465096719932No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCGCG260.1822259601906364No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC240.16820857863751051No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT240.16820857863751051No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.16820857863751051No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG240.16820857863751051No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC240.16820857863751051No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAGCG230.16119988786094758No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT210.1471825063078217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT210.1471825063078217No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCG200.14017381553125877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT200.14017381553125877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.13316512475469583No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT180.1261564339781329No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCATCTCG180.1261564339781329No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCGCTCGTCAGCT150.10513036164844407No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC150.10513036164844407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)